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Antibodies
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Date Published
Easy to Use and Gives Good Isolation
(1)
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Miltenyi Biotec
September 06, 2021
I used this to isolate CD8 positive cells from the NHP PBMCs and it takes 45 minutes.
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Decent Thermometer for Low Sensitivity Applications
(1)
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September 06, 2021
We are broadly interested in studying the mechanisms responsible for establishing and maintaining peripheral immune tolerance.
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Very Good Bench Fridge
(1)
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September 06, 2021
We are generally interested in studying the mechanisms responsible for peripheral immune tolerance.
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Hard to Optimize IL-22
(1)
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Thermo Fisher Scientific
September 03, 2021
I've tried many times with this antibody but never got a good staining.
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Very Good DNA Ladder from ThermoFisher
(1)
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Thermo Fisher Scientific
September 01, 2021
The ladder is very easy to use and doesn't require much optimization.
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Great for Measuring MYH11 Expression
(1)
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Santa Cruz Biotechnology, Inc.
September 01, 2021
Myosin is one of the highly conserved, ubiquitously expressed protein that interacts with Actin to generate the force for cellular movements...
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Great CD11c N410 Clone
(1)
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September 01, 2021
Our lab is interested in mechanisms of peripheral and central immune tolerance as it pertains to tumor and transplant immunology.
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Expensive yet Good Compensation Beads
(1)
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September 01, 2021
Great compensation beads to use for preparing single stain controls for flow cytometry.
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Antibody Gives Consistent Staining
(1)
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Thermo Fisher Scientific
September 01, 2021
We use this antibody regularly to stain antigen presenting cells from the thymus and the lymph nodes. It works great and the staining is ...
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Great Antibody for Flow Staining
(1)
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BioLegend
September 01, 2021
We use this antibody regularly to stain immune cells from the thymus and the lymph nodes. It works great and the staining is strong.
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Oral Gavage Tubes for Mice
(1)
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August 31, 2021
Our lab studies mechanisms of peripheral immune tolerance in the settings of tumor immunology and transplant tolerance.
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Brilliant Violet 421 Anti-mouse/human IL-5 Has Good Titration
(1)
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BioLegend
August 31, 2021
Used to identify IL-5 producing T cells and ILC2s in perigonadal fat following HFD.
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Really Good Centrifuge
(1)
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August 31, 2021
This mini centrifuge works great, we use it to pull down the small volume solutions.
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Good Price for Pipettes
(1)
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Evident
August 31, 2021
We use serological pippets routinely in the lab for solutions in the lab.
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Review of the Qiagen SARS-CoV-2 N1+N2 Assay Kit
(1)
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August 31, 2021
We sought to investigate the efficacy of the Qiagen SARS-CoV-2 N1+N2 Assay Kit in identifying samples containing SARS-CoV-2 viral RNA. Our ...
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Phospho-Paxillin (Tyr31) Antibody
(1)
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August 31, 2021
We used this product to check the phosphorylation level of paxillin protein. The antibody works well at the suggested dilution but gives ...
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Consistent DPBS 10X
(1)
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MilliporeSigma
August 31, 2021
This is a stable and sterile DPBS 10X. Have never had issues with contamination. Affordable.
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Highly Recommended Alexa Fluor 647 Anti-Mouse
(1)
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BioLegend
August 31, 2021
Antibody was used to identify IFNg producing CD8 and CD4 T cells.
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Great for Flow Staining with Biotin
(1)
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BioLegend
August 30, 2021
We use this regularly to stain with CCR7-Biotin for migratory dendritic cells from the lymph nodes. It works great and the staining is ...
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Great and Simple Medium
(1)
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MilliporeSigma
August 30, 2021
Our lab routinely use DMEM for collection of lymph node samples.
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Good Viability Dye
(1)
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Thermo Fisher Scientific
August 30, 2021
Good viability dye to get rid of dead cells. This is also compatible with PFA-based fixation and permeabilization for downstream ...
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Reliable CD8a Antibody for Flow Cytometry
(1)
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BioLegend
August 27, 2021
Reliable Antibody for flow analysis. Try 1:100 dilution in FACS buffer first. Titration might be needed for optimized results.
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Great CD3 Antibody for Flow
(1)
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BioLegend
August 27, 2021
Reliable Antibody for flow analysis. Try 1:100 dilution in FACS buffer first. Titration might be needed for optimized results.
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Solid Mini Prep Kit that Requires some Tuning
(1)
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QIAGEN - Sample to Insight
August 27, 2021
We broadly study the mechanisms responsible for establishing and maintaining immune tolerance in the periphery
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Dependable Phospho-SMAD3 Antibody for WB
(1)
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GeneTex
August 27, 2021
This antibody was used to detect p-Smad3 expression in PC-3 cells by western blot analysis.
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Amazing CD45 Antibody
(1)
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BioLegend
August 26, 2021
CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains.
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Very Bright Fluorophore and Good Antibody
(1)
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BioLegend
August 26, 2021
Reliable Antibody for flow analysis. Try 1:100 dilution in FACS buffer first. Titration might be needed for optimized results.
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Bright Fluorochrome Works Great
(1)
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BioLegend
August 26, 2021
One of the brightest flurochrome-strept conjugates. We routinely use this for our tetramer stainings for cell lines or primarty cells.
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Easy to Use PE/Dazzle 594 Anti-Mouse CD8b
(1)
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BioLegend
August 24, 2021
Easy to use. Titration at 1:400 worked well.
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Great for Measuring p120 Expression
(1)
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Santa Cruz Biotechnology, Inc.
August 24, 2021
The p120 catenin protein was originally identified in a screen for substrates of the Src tyrosine kinase as a 120kDa protein whose ...
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Reliable PE Anti-mouse/human CD11b
(1)
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BioLegend
August 24, 2021
Easy to use antibody. Good stain index for particular antigen and fluorophore. Cost-effective.
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Fast and Affordable Sequencing Service
(1)
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August 24, 2021
This service was used for validation of golden gate and Gibson assemblies to clone constructs for CRISPR guide libraries.
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Great PCR Machine
(1)
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Thermo Fisher Scientific
August 24, 2021
This is a 3x32 tube PCR machine, we use it for routine genotype PCR.
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Solid Eppendorf Centrifuge
(1)
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Eppendorf
August 24, 2021
We use it for centrifuging immune cells.
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Rainin P1000
(1)
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August 23, 2021
Our lab is interested in cellular mechanisms driving induction of peripheral immune tolerance. We are studying these mechanisms in the ...
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Rainin P200 Multi-channel
(1)
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August 23, 2021
Our lab is interested in cellular mechanisms driving induction of peripheral immune tolerance. We are studying these mechanisms in the ...
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Good, Affordable EDTA
(1)
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MilliporeSigma
August 23, 2021
EDTA solution that works well in MACS/FACS buffer for cell digestion and processing. Helps maintain cell viability through processing of ...
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Streptavidin Microbeads
(1)
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Miltenyi Biotec
August 23, 2021
Our lab is interested in mechanisms of peripheral immune tolerance in a murine model
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Great Formaldehyde
(1)
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Thermo Fisher Scientific
August 23, 2021
This is a 16% Formaldehyde solution that does not contain methanol that works well for fixing of cells for flow cytometry and ...
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Reliable Anti-mouse Ly6G Antibody
(1)
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BD Biosciences
August 23, 2021
We use this antibody regularly to stain immune cells from the thymus and the lymph nodes. It works great and the staining is strong.
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Easy to Use ACK Lysing Buffer
(1)
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August 23, 2021
This buffer is easy to use and affordable and reliably lyses RBCs. Use it primarily for lysis in spleen samples in mice.
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Great Antibody for Flow Staining
(1)
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BioLegend
August 23, 2021
We use this antibody regularly to stain epithelial cells from the thymus. It works great and the staining is strong.
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MHCII I-AB eF450
(1)
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August 20, 2021
Our lab is interested in mechanisms of peripheral and central immune tolerance as it pertains to tumor and transplant immunology.
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Dependable Anti-His Probe
(1)
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Santa Cruz Biotechnology, Inc.
August 20, 2021
To test the expression of the ADAR1 p150 WT-His plasmid generated by QuikChange II XL SDM Kit, HEK293 cells were transfected with this ...
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Cheap Alternative for Conical Tubes
(1)
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Thomas Scientific
August 20, 2021
15ml conical tubes. We used to use corning brand, which was slightly pricey. Thomas scientific has offered us a deal and these tubes are ...
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Great GFAP Antibody
(1)
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Abcam
August 20, 2021
This antibody was used to detect Myelin Basic Protein (MBP) expression in mice brain tissue (~250um thickness) by Immunofluorescence (IF).
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Simple and Easy DNA Ladder
(1)
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Thermo Fisher Scientific
August 20, 2021
I use this DNA ladder for genotyping, simple and easy.
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Great Antibody for Flow Staining
(1)
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BioLegend
August 20, 2021
We use this antibody regularly to stain migratory dendritic cells from the lymph nodes. It requires special 37C incubation for 30min, but ...
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Good 96 Well PCR Machine
(1)
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Thermo Fisher Scientific
August 20, 2021
We use it for routine genotyping.
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BV421 Anti-mouse CD4 for Easy Detection of CD4+ T Cells
(1)
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BioLegend
August 20, 2021
Bright and easily distinguishable!
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Cell Fractionation
Immunoassay
In Vitro Transcription
ChIP-Seq
Northern Blot
3D Imaging
RNAi
ELISPOT
PCR Cloning
Particle Analysis
Southern Blot
Protein Modification
DNA Analysis
Expression
Cell-Based Assay
Gene Editing
In Vivo Testing
Protein Labeling
Other
Species
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Human
Mouse
Rat
Non-Human Primate
Rabbit
Sheep
Hamster
Chicken
Bovine
Canine
Porcine
Horse
Monkey
C. elegans/Worm
Insect
Amoeba/Protozoa
Bacteria/Archaea
Arabidopsis
Yeast/Fungi
Squirrel
Fish
Virus
Other Invertebrate
Xenopus/Amphibian
Drosophila
Goat
Rhesus
Tissue
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Adipose
Aorta
Artery
Bladder
Blood
Blood Vessel
Bone
Bone Marrow
Brain
Breast
Bronchus
Cerebral Cortex
Colon
Cornea
Coronary Artery
Dermis
Duodenum
Ear
Embryo
Endometrium
Epidermis
Eye
Foreskin
Gingiva
Hair
Heart
Hippocampus
Hypothalamus
In Vivo
Intestine
Kidney
Large Intestine
Liver
Lung
Lymph Node
Mammary Gland
Mouth
Muscle
Ovary
Pancreas
Peripheral Blood
Peritoneum
Placenta
Prostate
Rectum
Retina
Skeletal Muscle
Skin
Small Intestine
Spinal Cord
Spleen
Stomach
Striatum
Synovium
Tail
Teeth
Testis
Thymus
Thyroid Gland
Tonsil
Trachea
Umbilical Cord
Umbilical Vein
Uterus
Vein
Cell Type
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Adipocyte
Astrocyte
B Cell
CD8+ T Cell
Competent Cells
Dendritic Cell
Embryonic Stem Cell
Endothelial Cell
Eosinophil
Epithelial Cell
Fibroblast
Glial Cell
Granulocyte
Helper T Cell
Hematopoietic Stem Cell
Hepatocyte
HUVEC
Induced Pluripotent Stem Cell
Keratinocyte
Leukocytes
Lymphocyte
Macrophage
Mesenchymal Stem Cell
Microglia
Microvascular Endothelial Cell
Monocyte
Myoblasts
Myocyte
Neuron
Neutrophil
NK Cell
NKT Cell
Oligodendrocytes
Osteoclast
PBMC
Platelets
Progenitor Cell
Red Blood Cell
Regulatory T Cells
Skeletal Muscle Cell
Smooth Muscle Cell
Splenocyte
Stem Cell
Stromal Cell
Synoviocyte
T Cell
Thymocytes
Tumor Cells
Cell Line
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HeLa
PC3 (PC-3)
HEK293
HEK293T (HEK-293T)
CHO
Jurkat
3T3
Caco-2
HepG2
THP-1
U2OS
PC12 (PC-12)
HL60 (HL-60)
A375 (A-375)
MOLT4 (MOLT-4)
MCF-7
A431
MDA-MB-231
A549
N2a
Daudi
HCT-116
Raji
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