Good Primary Antibody for Detecting MK2

October 19, 2021

IMCB
NTHU
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

MAPKAPK-2 Antibody

Catalog Number:

3042

Clone Number:

N/A

The primary antibody is used to detect the levels of MK2 protein in the cell.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Murine macrophage cells

Primary Incubation

1:1000, O/N, 4 deg

Blocking Agent

3% BSA TBST

Secondary Incubation

1:10000, 1hr, RT

Tertiary Incubation

N/A

Detection

Fluorescence

Results Summary

Two proteins band were observed without much background. The antibody is easy to use without many optimizations required.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to detect

The Bad

NIL

The Bottom Line

Easy to use and is highly recommended to those who need to detect MK2 protein.

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