Great Antibody for GFAP+ Astrocytes

Vascular Dysfunction
University of Heidelberg
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Dakocytomation

Product Name:

GFAP antibody

Catalog Number:

Z0334

Clone Number:

N/A

Info: Supplier Page 

GFAP antibody was used to label astrocytes in the brain. the labelling was quite efficient

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Postnatal mouse brain tissue-P12

Primary Incubation

1:300, 4° C overnight

Blocking Agent

10% NDS in PBST (0.5% TritonX 100)

Secondary Incubation

1:500, AF 488

Tertiary Incubation

none

Detection

Immunofluorescence

Results Summary

Specific staining for astrocyte labelling was observed in the postnatal brain.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specific staining, works even for thicker sections upto 100µm , no background

The Bad

None

The Bottom Line

efficient staining, without background

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