Biolegend
Aiolos
653205
8B2
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We study how tissue resident immune cells regulate tissue homeostasis. For this, it is important to visualise the immune cells of interest within their native tissues. Yet at first, we validate various antibodies for IF, using specific tissues as positive control(s).
Immunofluorescence
PLP fixed mouse (C57BL/6) spleen cryosections
1 in 50 overnigth at room temperature
PBS with 1% BSA, 0.3% Triton X, 2% mouse serum, 2% rat serum
N/A
Zeiss 780 confocal microscope
20 um spleen cryosections were stained for Aiolos (red), CD45 (green), and counterstained with DAPI (blue).
Excellent staining
None
Reliable staining of Aiolos in spleenic cryosections.