Biolegend
CD68
137037
FA-11
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We seek to unravel how tissue resident immune cells mediate tissue homeostasis. For us is very important to pin point the anatomical location of immune cells of interest within their native tissues. Yet at first, we validate various antibodies for IF, using specific tissues as positive control.
Immunofluorescence
PLP fixed mouse (C57BL/6) spleen cryosections
1 in 200 overnight at room temperature
PBS with 1% BSA, 0.3% Triton X, 2% mouse serum, 2% rat serum
N/A
20 um cryosections of murine spleen were stained for B220 (white), F4/80(green), and CD68 (red).
Reliable & reproducible staining of CD68
Cannot thing of anything
Excellent staining of CD68+ cells in murine cryosections.