Biolegend
PerCP/Cyanine5.5 anti-mouse CD326 (Ep-CAM) Antibody
118219
G8.8
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We used this antibody to distinguish tissue-resident cells from circulating populations.
Flow Cytometry
Mouse lymph node
1:50 dilution, stain at 37C for 30 minutes
Fc block
N/A
We observed good separation of antibody negative and positive staining populations in thelymph node. We further gated on CD326 populations to investigate tissue-resident populations of cells
Results are very consistent.
Reliable antibody for intravital labeling to distinguish tissue-resident cell populations.