CD326 Antibody Works Great

Pharmacology
KUMC
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Biolegend

Product Name:

PerCP/Cyanine5.5 anti-mouse CD326 (Ep-CAM) Antibody

Catalog Number:

118219

Clone Number:

G8.8

We used this antibody to distinguish tissue-resident cells from circulating populations.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lymph node

Primary Incubation

1:50 dilution, stain at 37C for 30 minutes

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow Cytometry

Results Summary

We observed good separation of antibody negative and positive staining populations in thelymph node. We further gated on CD326 populations to investigate tissue-resident populations of cells

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Results are very consistent.

The Bad

N/A

The Bottom Line

Reliable antibody for intravital labeling to distinguish tissue-resident cell populations.

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