BD Biosciences
BUV496 Mouse Anti-Human CD3
612940
UCHT1
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Our research aims at understanding how some CD4 T cells become autoreactive in melanoma patients treated with check point inhibitors. CD4 T cells are usually identified as CD3+ CD4+ cells. In this experiment, we used anti CD3 antibodies to identify T cells.
Flow Cytometry
PMA/ionomycin/Calcium-stimulated and unstimulated healthy donor's PBMCs
Surface staining with anti-CD3 BUV495 1/150 (20' at 4°C, in the dark)
None
Staining with L/D dye (20' at 4°C, in the dark).
Cells were fixed and permeabilized with a commercial kit to counterstain for cytokines.
UV laser on Symphony cytometer
CD3 T cells were readily identified, even after activation of the cells.
Bright even after stimulation of the cells
It is expensive.
Good option to add a channel to the panel.