gibco
ACK Lysing Buffer
A10492-01
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We study the mechanisms responsible for the establishment and maintenance of central and peripheral immune tolerance
Primary culture of mouse splenocytes
Mouse spleens
Be careful not to use more than the manufacturer's recommended amount
Effectively lyses red blood cells from pulverized mouse spleens to allow for culture or analysis of pure splenocytes/lymphocytes in downstream experiments
Works great, highly effective
None
Strongly recommended for any experiments requiring RBC lysis