Sadaf Atarod

Postdoctoral Associate

Boston University

Running 1% Agarose Gel
December 05, 2018
The aim of this experiment was to run DNA samples on 1% agarose gel. The TrackIt™ 1 Kb Plus DNA Ladder was recommended by ... read more
Detection of Low-Femtogram Proteins
November 02, 2018
The aim of this experiment was to detect proteins expressed at lower levels using the western blot method. This ultra-sensitive ... read more
Detection of Proteins Using Western Blot Method
November 01, 2018
The aim of this experiment was to detect proteins of interest using primary and secondary antibodies. The quick spray ... read more
Assess Migration of Cells Using p-Nitrophenyl Phosphate (PNPP)
October 26, 2018
The aim of this experiment was to determine the migratory capacity of cells by detecting the protein phosphatase activity. The ... read more
Detect Protein of Interest Using Anti-Rabbit Secondary Antibody
October 25, 2018
The aim of this experiment was to detect Caspase-3 protein. The primary antibody was rabbit-derived, hence this anti-rabbit ... read more
Reverse Transcribe Total RNA
October 24, 2018
The Taqman reverse transcription kit was used to reverse transcribe total RNA extracted from cells. The kit was recommended by ... read more
Identification of Antibody for Loading Control
October 23, 2018
The monoclonal anti-beta-actin was used to detect beta-actin as loading control in the western blot method. This primary antibody ... read more
Detection of Apoptosis by Assessing Caspase-3 State
October 22, 2018
The aim of this experiment was to assess apoptosis by determining caspase-3 cleavage. The antibody was present in the lab and ... read more
First-Strand Synthesis SuperMix Kit for RT-qPCR
October 09, 2018
The aim of this experiment was to quantify genes of interest in total RNA extracted from rat smooth muscle cells. The kit was ... read more
Real-time Quantitative PCR Using SYBR Green Master Mix
October 08, 2018
The aim of this project was to quantify RSP9 expression levels in our smooth muscle cells. The iTaq Universal SYBR Green Complex ... read more
To Transfer Proteins From An SDS-PAGE Gel
June 18, 2018
The aim of this experiment was to transfer proteins from an SDS-PAGE gel onto the membrane using the semi-dry method. In this ... read more
Detecting Apoptosis In Cells
June 12, 2018
The aim of this experiment was to detect apoptosis in cells exposed to 5% ethanol. The detection kit was recommended by colleagues... read more
Determining Apoptotic Cells Using Flow Cytometry
June 11, 2018
The aim of this experiment was to determine apoptotic cells via the detection of Annexin V and PI. The BD FACS Canto II was ... read more
Detecting Proteins Using Bradford Assay
June 07, 2018
The aim of this experiment was to measure the amount of protein extracted using the Bradford protein assay. The Spectramax2 plate ... read more
Isotype Control For PE/Cy7 Flurophore
July 07, 2017
The aim of this experiment was to use the PE/Cy7 isotype as a negative control for sorting PE/Cy7 CD31 positive cells in the mouse... read more

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A Fluorescence Assay Kit to Measures Live Cells

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The CellTiter-Fluor™ Assay kit was purchased to measure living cell number and to normalize relative gene expression. The results indicated that the assay ... read more

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