Detecting Apoptosis In Cells

Boston University
Center for Regenerative Medicine
Postdoctoral Associate

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Company:

Sigma-Aldrich (Now a Part of MilliporeSigma)

Product Name:

Annexin V-FITC Apoptosis Detection Kit

Catalog Number:

APOAF-20TST

The aim of this experiment was to detect apoptosis in cells exposed to 5% ethanol. The detection kit was recommended by colleagues.

Experimental Design and Results Summary

Application

Apoptosis Detection

Starting Material

Pulmonary Arterial Smooth Muscle Cells

Protocol Overview

Add 5 ul of abs to the cells 5 min prior to detection using flow cytometry.

Tips

Protect from light exposure.

Results Summary

Apoptosis was detected in cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Reliable.

The Bad

Expensive.

The Bottom Line

Reliable Annexin V- apoptosis detection kit.

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