Denville Scientific
HyGLO™ Quick Spray, HyGLO™ Quick Spray Chemiluminescent HRP Antibody Detection Reagent
E2400
Info: Browse Western Blot Reagents
The aim of this experiment was to detect proteins of interest using primary and secondary antibodies. The quick spray chemiluminescent was used to detect the protein. This reagent was recommended by colleagues.
Western Blot
Protein extracts run on an SDS-PAGE gel
Perform standard western blot method. Spray the chemiluminescent post-secondary antibody wash. The signal can be detected on autoradiography film.
Incubate for 1 minute with the chemiluminescent.
Proteins easily detected.
N/A
Very easy to use. No mixing required as it is a spray.
None
Sensitive to one pictogram of antigen.