First-Strand Synthesis SuperMix Kit for RT-qPCR

October 09, 2018

BlueskyReddit
Boston University
Center for Regenerative Medicine
Postdoctoral Associate

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Company:

Invitrogen

Product Name:

SuperScript III First-Strand

Catalog Number:

18080400

The aim of this experiment was to quantify genes of interest in total RNA extracted from rat smooth muscle cells. The kit was available in-house and recommended by colleagues.

Experimental Design and Results Summary

Application

RT-qPCR

Starting Material

Total RNA extracted from rat smooth muscle cells

Protocol Overview

The protocol was followed as per the manufacturer's guidelines.

Tips

Perform DNA digest when using TRIZOL total RNA extraction method.

Results Summary

cDNA was synthesized and used for RT-qPCR reaction.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Up to 5 ug of total RNA can be reverse transcribed in one reaction.

The Bad

The cDNA synthesis is cumbersome, multi-stepped.

The Bottom Line

It is a useful kit to use when a high concentration of total RNA is required to be reverse transcribed in one reaction.

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