Detect Protein of Interest Using Anti-Rabbit Secondary Antibody

October 25, 2018

Center for Regenerative Medicine
Boston University
Postdoctoral Associate

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Company:

Cell Signaling Technology

Product Name:

Anti-rabbit IgG, HRP-linked Antibody

Catalog Number:

7074

The aim of this experiment was to detect Caspase-3 protein. The primary antibody was rabbit-derived, hence this anti-rabbit secondary antibody was recommended by colleagues.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Protein extract

Primary Incubation

1:1000

Blocking Agent

5% non-fat milk

Secondary Incubation

Anti-rabbit secondary antibody at 1:2500

Tertiary Incubation

None

Detection

Chemiluminescence detection method

Results Summary

Caspase-3 protein was detected.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Specific

The Bad

Dilution is 1:2500, so more antibody will be used.

The Bottom Line

Reliable and easy-to-use secondary antibody.

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