Determining Apoptotic Cells Using Flow Cytometry

Boston University
Center for Regenerative Medicine
Postdoctoral Associate

Overall

Performance

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BD FACS Canto II

Catalog Number:

338960

The aim of this experiment was to determine apoptotic cells via the detection of Annexin V and PI. The BD FACS Canto II was recommended by colleagues.

Experimental Design and Results Summary

Application

Flow Cytometry

Protocol Overview

Turn the machine on first, before turning the computer on. Perform Fluidics Startup if you are first person using the machine and Fluidics Shutdown when you are the last person. There is no need to perform wash step between samples as it is automatically done.

Tips

Wash for 5 min with bleach and 5 min with water when experiment is completed.

Features Summary

Very easy to use.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Reliable, easy to use. Minimal maintenance.

The Bad

None

The Bottom Line

Simple to use flow cytometry machine. Performs compensation too.

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