Determining Apoptotic Cells Using Flow Cytometry

June 11, 2018

BlueskyReddit
Boston University
Center for Regenerative Medicine
Postdoctoral Associate

Overall

Performance

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BD FACS Canto II

Catalog Number:

338960

The aim of this experiment was to determine apoptotic cells via the detection of Annexin V and PI. The BD FACS Canto II was recommended by colleagues.

Experimental Design and Results Summary

Tips

Wash for 5 min with bleach and 5 min with water when experiment is completed.

DOI or PMID #

N/A

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Summary

The Good

Reliable, easy to use. Minimal maintenance.

The Bad

None

The Bottom Line

Simple to use flow cytometry machine. Performs compensation too.

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