To Transfer Proteins From An SDS-PAGE Gel

Boston University
Center for Regenerative Medicine
Postdoctoral Associate

Overall

Performance

Ease-of-Optimization

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Company:

Bio-Rad

Product Name:

Trans-Blot® SD Semi-Dry Transfer Cell

Catalog Number:

1703940

The aim of this experiment was to transfer proteins from an SDS-PAGE gel onto the membrane using the semi-dry method. In this method, low volumes of transfer buffer are utilized. This equipment was recommended by colleagues and available in the lab.

Experimental Design and Results Summary

Application

Semi-dry transfer of proteins

Protocol Overview

The BIORAD protocol is very precise and following it will yield result.

Tips

Run the transfer at 15V for 1.5 hrs

Features Summary

Very easy to use.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Reliable and easy to use. It is not a messy transfer.

The Bad

Takes a long time.

The Bottom Line

It is a very reliable equipment for transferring proteins from the SDS-PAGE gel.

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