Gurpanna Saggu

Postdoctoral Fellow

Brigham and Women's Hospital

Quick & Easy Viral Titer Estimate
May 07, 2018
Recently used the Clontech Lenti-X go six to qualify my supernatants for viral titer. They are super easy to use and interpret and... read more
The Best ELISPOT Reader Available
January 19, 2016
This is our new acquisition for reading ELISPOT plates. The most significant advantages are: a high zoom lens for seamless ... read more
An Alternative to Ficoll for Separating Mouse Cells
January 08, 2016
This product is used for isolating lymphocytes from mouse tissues including blood. It works on the principle of density gradient ... read more
A Superior Enzyme for Digesting Mouse Tissues
January 07, 2016
Liberase TL (thermolysin low) is a research grade enzyme that is used for dissociation of a broad range of tissues particularly ... read more
Pre-Synthesized mRNA for Cell Manipulation
January 05, 2016
Preparing good quality mRNA for transfection is difficult due to specific requirements of the polyA tail and mRNA instability. I ... read more
The Best Clone for Detecting Granzyme B
January 04, 2016
GranzymeB is expressed in cytotoxic T lymphocytes and NK cells after antigen activation. Granzyme B released by T cells causes ... read more
A Good Cell Proliferation Marker for In Vitro and Ex Vivo Studies
December 15, 2015
I used this antibody for staining Ki-67, a nuclear antigen that is expressed in cycling cells. The advantage is that you can stain... read more
Brilliant Violet Flow Antibody for CD25
November 19, 2015
CD25 or IL-2 receptor alpha is only expressed on Treg cells and transiently expressed on activated T cells. I have never performed... read more
Messy Staining for Class-II MHC
November 13, 2015
Class-II MHC is expressed on all antigen presenting cells including DCs, macrophages and B cells. The expression goes up during an... read more
Antibody to Detect B Cells
November 13, 2015
CD19 is expressed on all pro-B to mature B cells (during development) and follicular dendritic cells, but not plasma cells; thus ... read more
Bright Stain for Interferon Gamma
November 11, 2015
This antibody binds to mouse interferon gamma, a cytokine that is involved in T cell immunity and inflammation. Since, cytokines ... read more
Perhaps the Best Clone for Staining PD1
November 10, 2015
PD1 (Programmed Death receptor 1) is expressed on chronically activated and exhausted T cells. Hence, it is only expressed during ... read more
Best Antibody for Intracellular FoxP3 Staining
November 09, 2015
FoxP3 is expressed by regulatory T cells (Treg) in mice and is a master regulator of Treg function. Since FoxP3 is a transcription... read more
Anti-CD3 Antibody With Low Level Fluorescence
November 05, 2015
PERCP-Cy5.5 is not the best channel, but was the only one available in my staining panel. So, I decided to use it for a lineage ... read more
Myeloid Lineage Marker Staining
November 02, 2015
CD11b is expressed on several immune cells including macrophages and monocytes. I used this antibody for identifying CD11b high ... read more

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Simple, Sensitive Add-and-Read Viability Assay That's Easy to Multiplex

Wei Yang Sit

The Promega CellTiter-Blue Cell Viability Assayis used for cell viability assay. The workflow is about as simple as it gets: add one reagent directly to cells ... read more

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