BD Biosciences
Alexa Fluor 700 Mouse anti-Human Granzyme B
560213
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GranzymeB is expressed in cytotoxic T lymphocytes and NK cells after antigen activation. Granzyme B released by T cells causes apoptosis of target cells like tumors or foreign grafts. This antibody was used to measure granzyme B expression in poly-clonally activated CD8 T cells. The staining worked well with minimal background.
Flow Cytometry
Purified mouse CD8 T cells
1:200 in Perm/Wash buffer
FcR block
NA
Flow Cytometry - Alexa fluor 700 channel
The dot plots show good staining with minimal background. A lot of AF700 dyes bleed into APC channel, but this one was easy to compensate as seen in the dot plot measuring AF700 against APC.
None
Easy to use and compensate
A good antibody to stain functional cytotoxic T lymphocytes.