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Author Profile
Vincent Giacalone
Research Scientist
Biotech startup in stealth mode
In my role as a scientist I focus on developing novel immunotherapies by exploiting the underused potential of innate immune cells.
Articles by Vincent Giacalone
Reagent for Biotin–Protein Conjugation in Immunoassays
January 12, 2026
To prepare the product, reconstitute it to create a 10 mM stock solution. Next, add this solution to your protein of interest at a...
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Positive Control Stimulus for T-cell Activation
July 26, 2023
T-cell activation generally requires specific activation of the T-cell receptor. Antigen-independent activation can be achieved ...
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Reliable Antibody for Human CD3
June 16, 2023
This antibody targets the epsilon subunit of CD3. It offers robust signal in the PE channel and can be used in gating for T cells ...
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Reliable Live/Dead Stain for APC Cy7 Channel
May 31, 2023
This reagent labels dead cells as positive for flow cytometry. It is compatible with fixation but must not have any protein in ...
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Alternative Glutamine Supplement with Improved Stability
May 02, 2023
Many cell lines require glutamine supplementation in media, but it spontaneously degrades over time. The GlutaMax supplement has ...
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Non-Enzymatic Alternative to Trypsin
April 21, 2023
The Cellstripper product can be used to release adherent cells without any enzyme activity. This product is ideally suited to ...
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Reliable Culture Kit for Dendritic Cells
March 30, 2023
The ImmunoCult differentiation kit can be used to reliably differentiate human monocytes to dendritic cells.
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Reliable BCA Kit with Short Incubation
March 16, 2023
This kit is a variation of the standard BCA assay kit from ThermoFisher. Instead of a 30-minute incubation, this kit only requires...
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Thermo-Stable Alternative to Trypsin
March 03, 2023
Trypsin is a common reagent for detaching adherent cells, but must be stored frozen and activity can decline over time. The TrypLE...
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Convenient FBS Aliquots from Thermo Fisher
February 22, 2023
Most types of growth media for cell culture use FBS as an additive, but it can be a hassle to thaw an entire froze bottle and ...
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Excellent Alternative Cell Freezing Solution
February 03, 2023
Anyone who performs cell culture knows the importance of having a reliable way to freeze down cells. the standard solution of 10% ...
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Reliable Antibody for High MW Loading Control
January 11, 2023
I use this primary antibody to detect the protein vinculin as a loading control for western blots. It is a higher MW than GAPDH so...
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Product Review
Excellent Astrocyte Labeling with Low Background in Mouse Hippocampus
Ksenia Novototskaya
(2)
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We used this antibody to immunofluorescently label astrocytes in the mouse dorsal hippocampus following astrocyte-specific MOR knockdown. The signal was bright,...
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