Reliable Culture Kit for Dendritic Cells

March 30, 2023

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Biology
Research Scientist

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Quality of Results

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Company:

StemCell Technologies

Product Name:

ImmunoCult ACF Dendritic Cell Medium

Catalog Number:

10987

The ImmunoCult differentiation kit can be used to reliably differentiate human monocytes to dendritic cells

Experimental Design and Results Summary

Application

Cell culture

Starting Material

Monocytes from PBMC

Tips

The vials for the differentiation and maturation reagents look very similar, be careful not to switch them.

Results Summary

This kit contains media and supplement to differentiate monocytes to dendritic cells in under 1 week. The differentiated cells adopts an adherent, stretchy morphology.

DOI or PMID #

N/A

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Summary

The Good

Consistent differentiation outcome.

The Bad

Expensive, but no technical downsides.

The Bottom Line

This kit is a great way to generate dendritic cells in vitro. The product manual includes a schedule for when to change media, harvest cells are particular time points etc. Overall highly recommend.

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