Reliable Antibody for Human CD3

June 16, 2023

Biology
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Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE-conjugated anti-human CD3

Catalog Number:

981004

Clone Number:

SK7

Info:Supplier Page View Product Specs

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This antibody targets the epsilon subunit of CD3. It offers robust signal in the PE channel and can be used in gating for T cells in flow cytometry analysis.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PBMC

Primary Incubation

1:400

Blocking Agent

Fc receptor blocking solution

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

PE channel

Results Summary

This is a good antibody to use for gating on T cells. CD3 is highly expressed on T cells and PE is a bright fluorochrome so I use this antibody at a dilution of 1:400 without any significant compensation issues in adjacent channels.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Bright signal and compatible with fixation.

The Bad

None

The Bottom Line

Good antibody for gating on T cells.

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