Reagent for Biotin–Protein Conjugation in Immunoassays

To prepare the product, reconstitute it to create a 10 mM stock solution. Next, add this solution to your protein of interest at a molar ratio of 20:1. This ratio typically results in 3 to 5 biotin molecules binding to each target protein. Incubate the mixture at room temperature for 30 minutes. After incubation, perform a buffer exchange with the desired storage solution and store the conjugated material at 4°C. When investigating whether antigen-presenting cells are displaying cancer cell antigens on their surface, stain the cells with a biotinylated antibody that is specific to the target antigen. Complete the staining process by adding streptavidin-PE, and then analyze the cells using flow cytometry.