Non-Enzymatic Alternative to Trypsin

Results Summary

Similar to trypsin, add this reagent to your culture of adherent cells (i.e. 5 mL for a T-75 flask) and incubate until the cells detach. Because there is no enzyme activity this product may act much slower depending on the cells, but it is more gently for particularly sensitive cell types. It is advertised as compatible with media that contains serum, but results may be improved by gently rinsing cells with PBS first to remove traces of media.