Anti-GFAP antibodies are used in the immunodetection of the protein glial fibrillary acidic protein. In humans, the canonical protein has a reported length of 432 amino acid residues and a mass of 49.9 kDa. Its subcellular localization is in the cytoplasm. Up to 3 different isoforms have been reported for this protein. It is reported to be expressed in cells lacking fibronectin. A member of the Intermediate filament protein family, GFAP is known to be involved with Bergmann glial cell differentiation and extracellular matrix organization.
The GFAP marker can be used to identify Brain Astrocytes and Quiescent Hepatic Stellate Cells.* Synonyms for this target antigen include ALXDRD. GFAP gene orthologs have been reported in the mouse, rat, bovine, zebrafish, chimpanzee and chicken species. Over 7000 citations in the literature describe the use of GFAP antibodies in research. Western Blot is a widely used application for these antibodies. Immunofluorescence and Immunohistochemistry are also common applications.
*HuBMAP Human Reference Atlas v1.4