Deep tissue imaging with non-curing, refractive index matched SlowFade™ Glass. Cryo-preserved rat brain sections (100 µm thick), stained for tubulin (red) with Mouse Anti-Beta3-Tubulin (Cat. No. MA1-118) and GFAP (yellow) Rabbit Anti-GFAP (Cat. No. OPA1-06100). Targets were detected with Alexa Fluor™ Plus 594 Goat Anti-Mouse (Cat. No. A-11032) and Alexa Fluor™ Plus 647 Goat Anti-Rabbit (Cat. No. A-32733). Nuclei (Cyan) were stained with DAPI (Cat. No. D1306). Slides were mounted with SlowFade™ Glass non-curing Antifade Mountant (Cat. No. S36917) and imaged with a Zeiss LSM 710 confocal microscope using a Plan-Apochromat 63×/1.4 NA Oil immersion objective at a rate of 71 nm in the x and y dimensions and 110 nm in the z dimension, with a pixel size of 0.07 µm. Z-projections were generated using Zeiss™ Zen software.