Great GFAP Antibody for IF

August 09, 2021

Physical Medicine and Rehabilitation
National Taiwan University Hospital and National Taiwan University College of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher

Product Name:

Great GFAP Antibody

Catalog Number:

MA5-12023

Clone Number:

ASTRO6

This antibody was used to detect GFAP expression (astrocyte) in mice brain tissue (~250um thickness) by Immunofluorescence (IF).

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

mice brain (250um slice), dentate gyrus

Primary Incubation

1:100, 4℃, 2 day in SignalStain® Antibody Diluent .

Blocking Agent

10% normal goat serum, 1% triton-X100,2.5%DMSO,0.2% sodium azide in PBS

Secondary Incubation

1:200, Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488, 4℃, 2 day in SignalStain® Antibody Diluent .

Tertiary Incubation

None.

Detection

Alexa Fluor 633, zeiss LSM 880

Results Summary

Nice signal & permeability in 250um thickness tissue.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

High senesitivity, good permeability

The Bad

None.

The Bottom Line

We will continue to use this antibody.

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