Proteintech Group Inc
GFAP antibody
60190-1-Ig
40000381
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Primary microglia were isolated from mouse brain. Cells were kept in culture for a week followed by cell lysis and western blot to detect GFAP expression using this antibody.
Western Blot
Mouse microglia
Dilution 1:10,000 in 5% BSA in TBST for 16 h at 4'C
5% milk in TBST
1:100,000 in 5% milk in TBST for 2 h at 25'C
N/A
HRP chemiluminescence
We have used 3 different GFAP antibodies and this is the best working we found. It gives a clear band at ~50 kDa without any unspecific band.
Gives only a specific band without any background.
Very good antibody to detect GFAP expression using Western blot.