Great Astrocyte Marker for Feline Optic Nerve from Novus

Neuroscience
Wisconsin University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

GFAP antibody

Catalog Number:

NBP1-05198

Clone Number:

Polyclonal

Our research focuses on understanding a role played by glial cells in neurodegenerative diseases. The specific goal of this experiment was to quantify glial cell activation in the course of disease progression. This antibody demonstrated excellent staining for glial cells, in particular astrocytes, in the optic nerve.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Feline optic nerve tissues

Primary Incubation

Diluted at 1:1000, overnight at 4°C

Blocking Agent

10% normal donkey serum + 0.5% triton X + 0.1M PBS

Secondary Incubation

Alexa 488 conjugated anti-mouse IgG 1:500, 1 hr at RT

Tertiary Incubation

N/A

Detection

Fluorescence

Results Summary

Robust signals from the labeling for astrocytes was detected (green) in feline optic nerve tissues.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Highly specific with no background

The Bad

N/A

The Bottom Line

Very specific astrocyte markers for feline species and generates robust signals.

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