Good GFAP Antibody for IHC from Bioss

May 23, 2023

Afyon Kocatepe University

Overall

Quality of Results

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Company:

Bioss Inc.

Product Name:

GFAP Polyclonal Antibody

Catalog Number:

BS-0199R

Clone Number:

Polyclonal, 51-150/432

Info:Supplier Page View Product Specs

Browse Similar Products: GFAP Antibodies

I had good results using the standard citrate buffer pH 6.0 antigen method on formol-fixed paraffin-blocked tissues. I don't think any other application is needed.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Rat brain

Primary Incubation

1/50, RT, 2 hours

Blocking Agent

BSA, 1%

Secondary Incubation

Biotinylated Rabbit IgG, RT, 2 hours

Tertiary Incubation

Streptavidin-peroxidase, RT, 1 hours / AEC chromagen, 20', RT

Detection

ABC-peroxidase method

Results Summary

A successful GFAP antibody that can show astrocytosis in the central nervous system or is used in the detection of astrocytic tumors. sharply marking astrocytes and their fibrils. Legend: GFAP positive astrocytes.

DOI or PMID #

N/A

Additional Notes

Gill's hematoxylin as a background staining

Image Gallery

Summary

The Good

Specific and sharp

The Bad

Nope

The Bottom Line

Good working GFAP antibody.

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