Anti-GFAP Chicken Primary Antibody From Aves Labs

December 27, 2023

Physiology and Biophysics
State University of New York at Buffalo
Postdoctoral Researcher

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Company:

Aves Labs

Product Name:

Anti-Glial Fibrillary Acidic Protein Antibody

Catalog Number:

GFAP

Clone Number:

Polyclonal

We use the Anti-GFAP chicken primary antibody from Aves Labs in several applications: immunofluorescence, immunocytochemistry, and combined immunohistochemistry with in situ hybridization. While the species reactivity is broad (human, mouse, non-human primate, and rat), we tested this anti-GFAP antibody with mouse and human samples only.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse brain samples

Primary Incubation

Chicken anti-GFAP antibody from Aves Labs; 1:500; overnight incubation at 4°C in the dark

Blocking Agent

10% normal goat serum

Secondary Incubation

Alexa Fluor 488 goat anti-chicken IgG (1:1000, A-11039, Life Technologies of Thermo Fisher Scientific ); 2 h incubation at room temperature

Tertiary Incubation

N/A

Detection

Inverted confocal microscope Leica-Sp8 using LASX software

Results Summary

We use this antibody to visualize astrocytes for subsequent morphological assessments of brain astrocytes. Only minimal background staining has been detected. The antibody works excellently over a broad range of dilutions, from 1:1000 to 1:500. Image: Representative confocal images of GFAP staining in the mouse hippocampus (fixed frozen tissue), 63x magnification, zoom factor 1.0.

DOI or PMID #

35275429

Additional Notes

N/A

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Summary

The Good

This antibody consistently delivers outstanding results, requiring low dilutions. Its exceptional performance underscores its reliability for precise and reproducible experiments.

The Bad

While evaluating this antibody, we have found no drawbacks or issues.

The Bottom Line

Chicken anti-GFAP antibody from Aves Labs performance has been consistently reliable, providing excellent results across various experiments. This antibody stands out for its overall effectiveness without any discernible shortcomings.

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