Ahmad Al

Lab Technician

UMass

This CD13 Antibody From BD Biosciences was Dimmer Than Expected
May 15, 2024
Our lab uses CD13 as one of its core myeloid markers. Previously, we used CD13 PE (from the same vendor) and were happy with the ... read more
CD26 Antibody From BD Biosciences is Decent for Staining of Monocytes
May 15, 2024
Our lab uses CD26 in our T cell panel. It can be very useful in conditions where CD26 is lost, such as Sezary Syndrome / mycosis ... read more
CD5 Antibody From BD Biosciences Has a Good Resolution
April 17, 2024
CD5, a very important antigen in T cell development, is expressed at high levels in mature T cells. It is also seen on B cells, ... read more
CD33 Antibody From BD Biosciences Does Not Work as Expected
April 14, 2024
CD33 is typically positive on monocytes (bright) and neutrophils (intermediate/dim) but completely negative on lymphocytes. We ... read more
Multi Purpose CD10 Antibody From BD Biosciences
March 04, 2024
Our lab needs CD10 regularly. It is useful in identifying mature granulocytes and distinguishing them from the immature forms in ... read more
CD27 PE-Cy7 for Plasma Cells
March 01, 2024
Although CD27 is commonly used as a marker for lymphocytes (e.g., positive on medullary thymocytes and T cells, and also on ... read more
CD30 PE From BD Biosciences (for Reed-Sternberg Cells)
February 26, 2024
CD30 is never easy to detect by flow cytometry! This PE-conjugate is the best one I have found. It is not easy to optimize because... read more
Dim CD9 From BD Biosciences With Intermediate Positives
February 22, 2024
CD9, which is also known as p24 / tetraspanin-29 is often positive on mature B lymphocytes. However, it is upregulated in cancer, ... read more
An Average Anti-Lambda Monoclonal Antibody
February 07, 2024
Monoclonal anti-kappa and anti-lambda antibodies are not always of good quality. This is why many labs use polyclonal antibodies ... read more
Easy and Solid CD10 Staining With Antibody From BD Biosciences
February 06, 2024
CD10 is positive on mature neutrophils, but can also be seen on immature B cells (hematogones). We needed a good CD10 antibody ... read more
Clear Signal Was Obtained with CD5 PE From BD Biosciences
February 05, 2024
CD5 is used for identification of T cells, but also in the diagnosis of CLL, in which B cells express CD5 aberrantly. Typically, ... read more
CD13 - A Bright Marker for Myeloid Cells
April 07, 2023
Immunophenotyping of human hematolymphoid cells requires the combination of several antibodies to help identify the different ... read more
Acceptable CD7 Antibody from Beckman Coulter
April 07, 2023
Although CD7 is not a highly expressed antigen, it is an important one because of its pattern of expression. Typically, it is a ... read more
Useful Beads for Standardization, But...
February 17, 2023
Beckman Coulter made these beads for standardization of assays, and maybe of instruments as well. Standardization is of course a ... read more
Easy to Use Beads for Instrument QC
February 15, 2023
It is an absolute requirement that flow cytometers are optimized and quality-controlled daily before any samples are run to ensure... read more

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Excellent Astrocyte Labeling with Low Background in Mouse Hippocampus

Ksenia Novototskaya

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We used this antibody to immunofluorescently label astrocytes in the mouse dorsal hippocampus following astrocyte-specific MOR knockdown. The signal was bright,... read more

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