BD Biosciences
BD Horizon™ CD9 V450
658168
M-L13 (ASR)
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CD9, which is also known as p24 / tetraspanin-29 is often positive on mature B lymphocytes. However, it is upregulated in cancer, e.g., in B-lymphoblastic leukemia/lymphoma (B-LL). We use it to distinguish mature B cells from B-LL.
Flow cytometry
Blood and/or bone marrow
15-20 minutes in the fridge
5% FBS
None
Flow Cytometer equipped with Violet laser
The results on the B cells are dim, with unclear resolution from the negative population, and a smearing effect (see image); however, the B-LL cells tend to overexpress this marker, so we can still detect them (in conjunction with other antibodies of course).
N/A
Available on this channel (V450).
Dim signal - suboptimal resolution.
The antibody is just about OK, and it causes little compensation issues. Look around for alternatives before you buy this one.