Clear Signal Was Obtained with CD5 PE From BD Biosciences

Pathology
UMass
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

CD5 PE

Catalog Number:

340697

Clone Number:

Clone L17F12 (ASR)

Info:Supplier Page 

Browse Similar Products: Anti-CD5 Antibody Products

CD5 is used for identification of T cells, but also in the diagnosis of CLL, in which B cells express CD5 aberrantly. Typically, PE is too bright a dye, but in the diagnosis of CLL, it maybe a useful thing.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Human lymphocytes

Primary Incubation

15 minutes at room temp

Blocking Agent

None

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer (FL-2 channel)

Results Summary

The results of using this antibody made sense in that the T cells were nice and positive while the rest of the lymphocytes were negative. Other WBCs were also negative (as expected).

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good positive signal and good resolution.

The Bad

Very expensive and not concentrated enough.

The Bottom Line

This is a good but expensive antibody. Maybe because it is IVD.

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