BD Biosciences
BD™ PE Mouse Anti-Human CD26
340423
L272
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Our lab uses CD26 in our T cell panel. It can be very useful in conditions where CD26 is lost, such as Sezary Syndrome / mycosis fungoides. Using CD26 with PE was needed to ensure that we get a good, strong signal on positive T cells.
Flow cytometry
Blood and bone marrow
10-20 minutes @4°C
2% FBS
None
Blue (488nm) laser-equipped cytometer
Although the results on T cells were ok (as shown in the dot-plot, and on the single-parameter histogram - on which only the lymphocyte gate is displayed), we noticed a positive dim signal on monocytes. We did not expect this, and we are not sure why it happened.
N/A
Was positive on cells of interest.
Maybe non-specifically binging to monocytes.
This is a good antibody, but its use will depend on the lab's context of use. We think it was satisfactory for our own applications.