Easy to Use Beads for Instrument QC

February 15, 2023

UMass
Pathology
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Beckman Coulter

Product Name:

Flow-Check Pro Fluorospheres

Catalog Number:

A63493

It is an absolute requirement that flow cytometers are optimized and quality-controlled daily before any samples are run to ensure day-to-day consistency. Coulter's Flow-Check Pro Fluorospheres are an important part of this process. They are run daily to ensure optimal fluidics and laser alignment.

Experimental Design and Results Summary

Application

Flow Cytometry

Starting Material

N/A

Tips

Use fresh every day!

Results Summary

The results are very useful and are monitored in the software's Levey-Jennings plots. These can be vital in identifying errors on the fluidics side, or the laser/detectors. By running the beads and making sure that the cytometer is within specs, the lab will be able to run their precious samples without a worry.

DOI or PMID #

N/A

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Summary

The Good

Easy to use (straight out of the bottle). No dilution required.

The Bad

None

The Bottom Line

This is a very important product that every lab using Coulter machines should use in their daily set up. It is easy to use and no dilution into a buffer is required. The peaks in the plots show how tight the CV% are on the cytometer.

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