BD Biosciences
BD Pharmingen™ APC Mouse Anti-Human CD13
557454
WM15
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Our lab uses CD13 as one of its core myeloid markers. Previously, we used CD13 PE (from the same vendor) and were happy with the results. We had to switch to CD13 APC. Unfortunately, it did not perform in the same way.
Flow cytometry
Blood and bone marrow
10-20 minutes @4°C
2% FBS
None
Red (633nm) laser-equipped cytometer
The monocytes and granulocytes (both of which are typically positive for CD13) appeared positive, but with a much lower stain index and signal to noise ratio compared to what we are used to with CD13 PE. The antibody works (as shown in the plot) but it is not easy to resolve the dim positive populations.
N/A
Easy to titrate.
Relatively poor resolution.
The best advice I can give is to try a different antibody/clone if the signal you are getting is dim.