Julia Jackson

Research Associate
UCSF

Articles by Julia Jackson

  • Assay Kit/Reagent User Review

    Interesting RNA Isolation Kit

    March 30, 2018
    We purchased this kit for isolating RNA from cultured lung slices (both mouse and human) because we were struggling with getting a... read more
  • Equipment User Review

    High Performance For Precision Slices

    March 29, 2018
    We use this vibratome for making our precision cut lung slices (PCLS). We use both human and mouse slices in various culture ... read more
  • Antibody User Review

    Labeling CD64 Positive Cells for Flow Cytometry

    March 08, 2018
    This is a reliable antibody for labeling CD64 positive cells in mice. We use it to differentiate macrophage populations in mouse ... read more
  • Assay Kit/Reagent User Review

    Easy Viability Staining For Flow Cytometry

    February 27, 2018
    Sytox Blue staining is an easy and fast way to stain for viability in flow cytometry experiments. Instead of adding it with ... read more
  • Antibody User Review

    Reliable CD45 Flow Cytometry Staining

    February 22, 2018
    For most of our experiments, we look to exclude immune cells from our sorted cells because they're so auto fluorescent. We use ... read more
  • Antibody User Review

    Epcam Staining for Flow Cytometry

    July 24, 2017
    Usually when we do flow experiments, we are looking to isolate whole epithelial populations and sub-populations. Epcam is a great ... read more
  • Antibody User Review

    Easy Staining Of Immune Cells In Flow Samples

    July 21, 2017
    Generally, we want to sort for epithelial cell populations within the lung. Having immune cells in our prep makes this tricky, ... read more
  • Assay Kit/Reagent User Review

    Getting Rid Of Red Blood Cells

    June 07, 2017
    We use this pre-made red blood cell lysis buffer during our mouse and human preps for flow cytometry. Having a pre-made solution ... read more
  • Equipment User Review

    Bio-Rad Thermal Cycler

    May 31, 2017
    We have two of these thermal cyclers-one was purchased a few years ago, and one was purchased about a year ago. We use these for ... read more
  • Antibody User Review

    Alexa Fluor 488 Anti-rabbit Secondary Antibody

    May 30, 2017
    We use this secondary in combination with a variety of different antibodies in both cytospins and frozen sectioned tissue, namely ... read more
  • Assay Kit/Reagent User Review

    Dispase For Tissue Digests

    May 26, 2017
    We do a lot of whole lung mouse and human flow sorts, so we use this dispase as the base for our tissue digests. We isolate a ... read more
  • Assay Kit/Reagent User Review

    Embedding Mouse Lungs

    May 25, 2017
    We embed a lot of mouse and human tissue, namely lungs. We do a lot of immunofluorescence and choose to use frozen tissue because ... read more
  • Assay Kit/Reagent User Review

    Super Quick cDNA

    May 24, 2017
    We use this kit in conjunction with Bio-Rad's sso sybr green mix and the CFX connect. Standard qPCR from RNA isolated from ... read more
  • Assay Kit/Reagent User Review

    Easy Genotyping

    May 23, 2017
    We use this for all of our genotyping-usually ear clips that have been digested in Sigma extraction and neutralization solutions. ... read more
  • Antibody User Review

    Anti-Prosurfactant Protein C (proSP-C) Antibody

    May 22, 2017
    We use this antibody on both mouse organ sections and cytospins. The photo is of alveolar type two cells that were sorted, thrown ... read more
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