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Author Profile
Julia Jackson
Research Associate
UCSF
Articles by Julia Jackson
Assay Kit/Reagent User Review
Interesting RNA Isolation Kit
March 30, 2018
We purchased this kit for isolating RNA from cultured lung slices (both mouse and human) because we were struggling with getting a...
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Equipment User Review
High Performance For Precision Slices
March 29, 2018
We use this vibratome for making our precision cut lung slices (PCLS). We use both human and mouse slices in various culture ...
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Antibody User Review
Labeling CD64 Positive Cells for Flow Cytometry
March 08, 2018
This is a reliable antibody for labeling CD64 positive cells in mice. We use it to differentiate macrophage populations in mouse ...
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Assay Kit/Reagent User Review
Easy Viability Staining For Flow Cytometry
February 27, 2018
Sytox Blue staining is an easy and fast way to stain for viability in flow cytometry experiments. Instead of adding it with ...
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Antibody User Review
Reliable CD45 Flow Cytometry Staining
February 22, 2018
For most of our experiments, we look to exclude immune cells from our sorted cells because they're so auto fluorescent. We use ...
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Antibody User Review
Epcam Staining for Flow Cytometry
July 24, 2017
Usually when we do flow experiments, we are looking to isolate whole epithelial populations and sub-populations. Epcam is a great ...
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Antibody User Review
Easy Staining Of Immune Cells In Flow Samples
July 21, 2017
Generally, we want to sort for epithelial cell populations within the lung. Having immune cells in our prep makes this tricky, ...
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Assay Kit/Reagent User Review
Getting Rid Of Red Blood Cells
June 07, 2017
We use this pre-made red blood cell lysis buffer during our mouse and human preps for flow cytometry. Having a pre-made solution ...
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Equipment User Review
Bio-Rad Thermal Cycler
May 31, 2017
We have two of these thermal cyclers-one was purchased a few years ago, and one was purchased about a year ago. We use these for ...
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Antibody User Review
Alexa Fluor 488 Anti-rabbit Secondary Antibody
May 30, 2017
We use this secondary in combination with a variety of different antibodies in both cytospins and frozen sectioned tissue, namely ...
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Assay Kit/Reagent User Review
Dispase For Tissue Digests
May 26, 2017
We do a lot of whole lung mouse and human flow sorts, so we use this dispase as the base for our tissue digests. We isolate a ...
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Assay Kit/Reagent User Review
Embedding Mouse Lungs
May 25, 2017
We embed a lot of mouse and human tissue, namely lungs. We do a lot of immunofluorescence and choose to use frozen tissue because ...
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Assay Kit/Reagent User Review
Super Quick cDNA
May 24, 2017
We use this kit in conjunction with Bio-Rad's sso sybr green mix and the CFX connect. Standard qPCR from RNA isolated from ...
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Assay Kit/Reagent User Review
Easy Genotyping
May 23, 2017
We use this for all of our genotyping-usually ear clips that have been digested in Sigma extraction and neutralization solutions. ...
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Antibody User Review
Anti-Prosurfactant Protein C (proSP-C) Antibody
May 22, 2017
We use this antibody on both mouse organ sections and cytospins. The photo is of alveolar type two cells that were sorted, thrown ...
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Product Review
Efficient and Cost Friendly SYBR Green Mastermix for qPCR
Parijat Sarkar
I study the transcription of endogenous genes regulated by transcription factors, and it's crucial to quantify their expression levels accurately. To achieve ...
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