Invitrogen Antibodies by Thermo Fisher Scientific
Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488
a21206
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We use this secondary in combination with a variety of different antibodies in both cytospins and frozen sectioned tissue, namely lung and trachea. We do a lot of IF and this is the only brand of secondary antibody we use right now. This review can speak for most of the secondary antibodies in this group-they all work very well for us.
Immunofluorescence
Frozen OCT embedded mouse or human tissue, cytospin of sorted cells, cultured cells
Variable, 4 degrees overnight
5% BSA, HS, triton, PBS
1:1000 one hour room temp
N/A
Fluorescent scope
This secondary provides a reliable flourescence at 1:1000 in just an hour incubation. Works well with variety of cells-cultured, primary cytospins, and frozen fixed tissues.
Reliable, clear
Nothing!
This whole collection of secondaries works phenomenally and we don't use anything else.