Leica Biosystems
VT1000 S Vibrating blade microtome
14047235613
We use this vibratome for making our precision cut lung slices (PCLS). We use both human and mouse slices in various culture conditions and assess outcomes. This vibratome helps us get from a full chunk of tissue down to a manageable slice somewhere in the range of 300-600um. We use this particular vibratome because it can cut multiple sections and is much more user friendly than other vibratomes we have access to. We need precision with these slices and this machine gives us the security that we will get slices of uniform thickness and good morphology.
If you plan on culturing your slices, play around with the agarose % and source a bit. We use a low melt agarose at 2%, but this will depend on how thick you need to cut and what type of tissue it is.
N/A
Precise and automated
Nothing
Super consistent, takes the human error out of using a vibratome, very clear and easy to adjust.