Easy Viability Staining For Flow Cytometry

UCSF
Medicine
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

SYTOX™ Blue Dead Cell Stain, for flow cytometry

Catalog Number:

S34857

Sytox Blue staining is an easy and fast way to stain for viability in flow cytometry experiments. Instead of adding it with antibodies and incubating, you can just spike it into the final sort buffer. This saves time, especially if you're only staining for viability.

Experimental Design and Results Summary

Application

Flow Cytometry

Starting Material

Mouse single cell suspension

Protocol Overview

Follow your flow prep protocol until you have a single cell suspension and then resuspend at about 10 million cells per ml in your sort buffer. Spike in Sytox Blue at 1:1000 and start your flow!

Tips

Super easy to use-no incubation time!

Results Summary

Clear and fast separation of live and dead cells. Ability to keep staining as cells die if you have a large sample or are sorting for a long time.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Simple, quick

The Bad

Nothing!

The Bottom Line

Quick, easy, low maintenance viability assay for flow!

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