Nina Flerin

PhD Candidate
Albert Einstein College of Medicine

Articles by Nina Flerin

  • Antibody User Review

    Anti Mouse CD8a For Flow Cytometry

    February 07, 2017
    In our lab we work with different lentiviral constructs. One of the constructs we use to transduced human CD8+ T cells has a ... read more
  • Assay Kit/Reagent User Review

    Miniprep Kit To Isolate Plasmid DNA From Bacterial Cultures

    February 06, 2017
    In our lab we routinely perform molecular cloning to generate TCR constructs for lentiviral expression. In this process we use the... read more
  • Antibody User Review

    Human a/b TCR antibody for FACS

    December 16, 2016
    To study different TCRs cloned from CD8 T cells from HIV infected patients we transduced TCR deficient cells with lentiviral ... read more
  • Antibody User Review

    Anti Human MIP-1beta Antibody For Flow Cytometry

    December 08, 2016
    We use this antibody to stain primary human CD8 T cells following stimulation to compare activation potential of cells isolated ... read more
  • Antibody User Review

    Anti Human IFNgamma Antibody

    December 05, 2016
    We use this antibody to stain human CD8 T cells following stimulation using various conditions to determine activation capacity of... read more
  • Antibody User Review

    Anti Human Granzyme B Antibody

    December 02, 2016
    This antibody was used to stain human CD8 T cells following a 12h stimulation with PMA and Ionomycin as well as other activation ... read more
  • Antibody User Review

    Antibody For Staining Of Newly Formed Perforin

    December 01, 2016
    This antibody was used for intracellular staining of human CD8+ T cells upon 12h stimulation with PMA and ionomycin. The antibody ... read more
  • Assay Kit/Reagent User Review

    Antibody Biotinylation Kit

    November 30, 2016
    We used this kit to biotynilate an antibody to be used in FACS experiments to detect cells that are expressing HIV gp120. The ... read more
  • Antibody User Review

    Anti human CD28 for T cell activation

    November 10, 2016
    We used this antibody for activation and expansion of human CD8 and CD4 T cells. We used this antibody in combination of human ... read more
  • Assay Kit/Reagent User Review

    Human CD3/CD28 T Cell Activator Beads

    November 09, 2016
    We tested these beads for activation and expansion of human CD8 and CD4 T cells. Activated cells are later used for transduction ... read more
  • Assay Kit/Reagent User Review

    Mycoplasma Detection Kit

    November 08, 2016
    Our lab does a lot of cell culture with cell lines as well as primary human cells. Because it is crucial to avoid mycoplasma ... read more
  • Antibody User Review

    HLA-A2 Antibody For FACS

    November 07, 2016
    The general aim of my research is to investigate the role of TCRs in the function of CTLs. Because TCRs are specific not only for ... read more
  • Assay Kit/Reagent User Review

    Reagent To Increase Lentiviral Transduction In Primary Cells

    November 04, 2016
    we use this reagent to help achieve high transduction efficiency of human primary T cells. It has lower toxicity than polybrene ... read more
  • Antibody User Review

    HLA-B27 antibody for FACS

    November 03, 2016
    The general aim of my research is to investigate the role of TCRs in the function of CTLs. Because TCRs are specific not only for ... read more
  • Assay Kit/Reagent User Review

    Maxiprep Kit

    November 02, 2016
    We need high amounts of plasmid DNA to make lentiviruses to use in our research on TCRs. We selected this product because it is ... read more
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