Flow Staining of In Vitro Translation Activity of GM-CSF mRNA Vaccine

Research and Development Department
CNBG-Virogin Biotech (Shanghai)
Research Scientist

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Company:

Sino Biological

Product Name:

Recombinant Anti-GM-CSF/CSF2 Antibody, Rabbit Monoclonal

Catalog Number:

51048-R017

Clone Number:

N/A

This is used for in vitro translation activity detection. Cells transfected for 24h were collected and the samples were stained with this antibody, and then a PE secondary antibody was used. The positive percentage of cells in the transfected samples was about 99%, and the effect was very good

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

293T cell

Primary Incubation

1:500,1h,RT

Blocking Agent

5% Goat serum

Secondary Incubation

1:100,30min

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

This is the sample we received after transfecting the cells for 24h. After staining with this antibody, we continued to use a PE secondary antibody. Each column is a sample, and these two rows are two double pores. Antibody titer is very high, we are 1:500 detection, 1:300 and 1:500 for us to see the percentage difference is not big, maybe different dilution ratio has the biggest impact on the MFI value.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Antibody titer is high, specificity is strong, and repeated freezing and thawing twice does not affect the result.

The Bad

N/A

The Bottom Line

Antibodies are a bit expensive but very effective. Highly recommended.

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