BD Pharmingen™
Alexa Fluor® 700 Mouse anti-Human Granzyme B
561016
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This antibody was used to stain human CD8 T cells following a 12h stimulation with PMA and Ionomycin as well as other activation methods. We wanted to see the differences in activation between cells isolated from different donors expressing different TCRs.
Flow Cytometry
Human CD8 T cells
1:100, overnight, 4C
None
Flow cytometry, Alexa Fluor 700 channel
Antibody gives us great staining for GranzymeB. The results are consistent and with minimal background.
N/A
Great Staining, reasonable dilution
Great antibody to use for intracellular staining of human CD8 T cells