OZ Biosciences
LentiBlast Kit
LB00500
We use this reagent to help achieve high transduction efficiency of human primary T cells. It has lower toxicity than polybrene which is traditionally use for to enhance transduction.
Lentiviral transduction of primary human T cells
Human CD8+ T cells
Add 0.5ul of reagent A + 0.5ul of reagent B into each well of a 24 well plate containing 0.5ml of cells in media per well. Add lentivirus, spin for 1h.
Titrate reagents A and B for different cell types according to the protocol's instructions. 0.5uL of each worked the best for us.
Transduction efficiency was doubled when using lentiblast compared to Polybrene. Cell viability also improved
None
Increased transduction efficiency and cell viability
Great reagent for increasing lentiviral transduction efficiency in primary human T cells.