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Brilliant Violet 421™ anti-human Perforin Antibody
353307
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This antibody was used for intracellular staining of human CD8+ T cells upon 12h stimulation with PMA and ionomycin. The antibody works well.
Flow Cytometry
Human CD8+ T cells
1:50, overnight, 4C
None
Flow Cytometry, PacificBlue channel
Staining is not dramatic but we do see a consistent upregulation in perforin in cells stimulated with PMA and ionomycin compared to cells left unstimulated (medium only).
NA
Consistent results
Low intensity
Good antibody to use for intracellular staining of newly formed perforin.