Anti Human MIP-1beta Antibody For Flow Cytometry

Microbiology & Immunology
Albert Einstein College of Medicine
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

PE Mouse Anti-Human MIP-1β

Catalog Number:

550078

We use this antibody to stain primary human CD8 T cells following stimulation to compare activation potential of cells isolated from different donors using different stimulation conditions. We chose this antibody because we trust this company to produce quality antibody for use in flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human CD8 T cells

Primary Incubation

1:100 dilution, overnight, 4C

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry, PE channel

Results Summary

We see a nice shift in the fluorescence intensity in the stimulated cells when staining with this antibody as shown in the figure attached. Minimal background staining.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good staining

The Bad

None

The Bottom Line

This is a good antibody to use for MIP-1beta intracellular staining.

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