HLA-A2 Antibody For FACS

Microbiology & Immunology
Albert Einstein College of Medicine
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

FITC Mouse Anti-Human HLA-A2

Catalog Number:

551285

The general aim of my research is to investigate the role of TCRs in the function of CTLs. Because TCRs are specific not only for the peptide but also for the HLA type of the target cells, we needed to screen individuals to recruit donors who were HLA-A2 positive. We used this antibody for a primary screen of our donors.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

1:100, 30min, 4C

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry, FACS

Results Summary

Good, clean staining for HLA-A2. Low background in the negative control

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Good staining, low background

The Bad

none

The Bottom Line

Great antibody to stain for HLA-A2

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