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Author Profile
Maria Carmela Speranza
postdoc
BWH/HMS
Articles by Maria Carmela Speranza
Antibody User Review
Human Anti-Ki67 Antibody
June 14, 2016
Glioma cell line was used to induce flank tumors in athymic mice. Tumors were fixed, sectioned, blocked and stained with this ...
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Antibody User Review
Anti-CD31 Immunohistochemistry Antibody
June 13, 2016
Glioma cell line was used to induce flank tumors in athymic mice. Tumors were fixed, sectioned, blocked and stained with this ...
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Antibody User Review
hTIM-3 Antibody Review
June 10, 2016
This antibody was used to stain human PBMC cell lines after OK3 treatment. This is a good, but not excellent antibody.
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Antibody User Review
Anti-Mouse CD3 PECy7
May 16, 2016
This is a good anti-mouse CD3 antibody. The experiments were performed using mouse splenocytes stimulated in vitro for 3 days with...
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Antibody User Review
Good Anti-Mouse CD69 Antibody
May 13, 2016
Good antibody anti-mouse CD69. The experiments were performed using mouse splenocytes stimulated in vitro for 3 days with 0.5ng ...
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Antibody User Review
Good Anti-PDL1 for Immunohistochemistry
May 10, 2016
This antibody gives good detection of anti-PDL1 in mouse model. Poor cross-reactivity and background.
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Antibody User Review
Anti-Mouse PDL1 for Western Blot
May 09, 2016
This is a good anti-mouse CD274 antibody. Clear membrane, one single band.
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Assay Kit/Reagent User Review
ELISA mIFN beta
May 04, 2016
This IFN-beta ELISA kit by BioLegend is easy to use and fast.
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Antibody User Review
Cell Viability Kit for FC
March 01, 2016
This is a good and easy to use cell viability staining kit for flow cytometry.
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Assay Kit/Reagent User Review
RTK Pathway
February 26, 2016
This is a nice kit to detect multiple kinase of the RTK path. It is easy to use and with a good layout.
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Antibody User Review
Perfect Anti-Human CD3
February 19, 2016
This antibody was used to investigate the amount of T cells after PBMCs extraction. The results were perfect and the optimization ...
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Antibody User Review
Works in FACS, but Needs High Concentration
February 18, 2016
This antibody was used on human PBMC cells stimulated in vitro with OKT3 for 2 and 3 days. For the optimization I used multiple ...
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Antibody User Review
Anti-Mouse PDL1
February 17, 2016
This antibody was used on tumor mouse cell lines. It is a good anti-mouse PDL1 antibody - easy to optimize and gives small ...
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Antibody User Review
Checking for Non-Viable Cells
January 20, 2016
Working with viruses, it is always useful to check cell viability. Instead of doing a separate experiment every time, it is really...
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Product Review
Efficient and Cost Friendly SYBR Green Mastermix for qPCR
Parijat Sarkar
I study the transcription of endogenous genes regulated by transcription factors, and it's crucial to quantify their expression levels accurately. To achieve ...
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