Abcam
Anti-Ki67 antibody (ab15580)
ab15580
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Browse Similar Products: Ki67 Immunofluorescence Antibodies
Glioma cell line was used to induce flank tumors in athymic mice. Tumors were fixed, sectioned, blocked and stained with this antibody (1:100). Anti-Ki67 for human gives nice staining, also for fluorescence, but there is some background.
Immunohistochemistry
Human glioblastoma flank tumor - nude mice
O/N at 4C
Donkey serum
Alexa anti-rabbit R488 - 2h RT
N/A
Confocal microscope
1. block with donkey serum - 1h at RT 2. wash 3x - 10min each 3. incubate O/N at 4C with anti-Ki67 1:1004. wash 3x - 10min each 4. incubate 2h RT with the secondary antibody 5. wash 3x - 10min each
None
Works good in immunofluorescence
Some background present.
Nice antibody