Anti-Mouse PDL1

Neurosurgery
BWH/HMS
postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Horizon

Product Name:

PE Rat Anti-Mouse CD274

Catalog Number:

558091

This antibody was used on tumor mouse cell lines. It is a good anti-mouse PDL1 antibody - easy to optimize and gives small background.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Tumor mouse cell lines: GL261

Primary Incubation

30 min

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

PE

Results Summary

Sample has been washed three time with washing buffer before and after incubation. 600000 cells were collected and incubated with the 100 ul of antibody (1:50). An IgG2a isotype has been used as control.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Little background

The Bad

Relatively high concentration

The Bottom Line

Good monoclonal antibody for PDL1 in mouse. Tested only for flow cytometry.

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