Abcam
Anti-CD31 antibody
ab28364
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Glioma cell line was used to induce flank tumors in athymic mice. Tumors were fixed, sectioned, blocked and stained with this antibody (1:100). Good staining was achieved with human anti-CD31
Immunofluorescence
Human glioblastoma flank tumor - nude mice
O/N at 4C
Donkey serum
Alexa anti-rabbit R594 - 2h RT
N/A
Confocal microscope
1. Block 1h at RT with a donkey serum 2. wash 3 times - 10 min each 3. incubate O/N with anti-CD312. wash 3 times - 10 min each 4. incubate 2h at RT with anti-rabbit R5944. Wash 3 times - 15 min each
None
Easy staining
Need O/N staining
Good antibody - recommended for IHC (fluorescence)